Pharmacological investigation of antioxidant and anti-inflammatory activities of leaves and branches extracts from Plinia cauliflora (Jaboticaba). (15th November 2021)

• Record Type: Journal Article
• Authors: de Lima Paula, Priscila; de Oliveira Lemos, Ari Sérgio; Campos, Lara Melo; Ferreira, Thayná Gomes; Freitas de Souza, Thalita; Queiroz, Lucas Sales; Machado Resende Guedes, Maria Clara; Martins, Mário Machado; Goulart Filho, Luiz Ricardo; Macedo, Gilson Costa; Tavares, Guilherme Diniz; Rocha, Vinícius Novaes; Leite Denadai, Ângelo Márcio; Fabri, Rodrigo Luiz
• Is Part Of: Journal of ethnopharmacology. Volume 280(2021)
• Publication Date: 2021-11-15
• Subjects: Plinia cauliflora -- Jaboticaba -- Polyphenols -- Free radicals -- Nitric oxide -- Ear edema; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2021.114463
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Among all native Brazilian plant species, Plinia cauliflora (DC.) Kausel (Jaboticaba), is well known for producing "superfruits", due to their high phenolic content and antioxidant property. The fruit has astringent characteristics, and it is popularly known for the treatment of diarrhea, rash, and intestinal inflammation. However, there are only a few studies on the use of leaves and branches of this species in the literature, mainly to treat oxidative stress and inflammation. Aim of the study: The present study aimed to investigate the antioxidant and anti-inflammatory potential of leaves and branches extracts from P. cauliflora . Material and methods: The phytochemical analysis of P. cauliflora extracts was performed by the total phenolic, flavonoid, and tannin dosage method. Moreover, the compounds were identified by HPLC-MS-Q-TOF. Antioxidant capacity was determined by DPPH, β -carotene/linoleic acid system, MDA formation, and phosphomolybdenum assays. In vitro and in vivo anti-inflammatory activities of P. cauliflora were evaluated by the reduction of nitric oxide in the J774A.1 cell line and inhibition of ear edema in mice, respectively. Results: The ethanolic extract of the leaves exhibited greater flavonoid content whereas the ethanolic extract of the branches showed higher tannins content. Twenty-two and seventeen compounds were identified by HPLC-MS-Q-TOF in the leaves and branches, respectively, being tellimagrandin I, castalagin, and valoneic acid dilactone reported for the first time in P. cauliflora . The antioxidant potential of extracts was confirmed through different oxidation pathways from oxidizing radicals, which might be related to the presence of phenolic compounds. For the anti-inflammatory assay, the leaves and branches extracts showed promising results, with a reduction of nitric oxide ear edema inhibition around 95% and 80%, respectively. Conclusions: Herein, the great biological potential of leaves and branches extracts from P. cauliflora was highlighted. These parts of the plant are underused and poorly reported in the literature, especially for the antioxidant and anti-inflammatory activities. Graphical abstract: Image 1 Highlights: Leaves and branches of Plinia cauliflora are less portrayed in the literature. Tellimagrandin I reported for the first time in P. cauliflora leaves. Valoneic acid dilactone and castalagin reported for the first time in extracts. Leaves and branches extracts showed in vitro high antioxidant potential. Leaves and branches extracts reduced nitric oxide concentration and ear edema.