Description of Devosia faecipullorum sp. nov., harboring antibiotic- and toxic compound-resistant genes, isolated from poultry manure. Issue 7 (21st July 2021)

• Record Type: Journal Article
• Authors: Lin, Shih-Yao; Tsai, Chia-Fang; Hameed, Asif; Tang, Yu-Shan; Young, Chiu-Chung
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 71:Issue 7(2021)
• Publication Date: 2021-07-21
• Subjects: average nucleotide identity (ANI) -- Devosia chinhatensis -- Devosia faecipullorum; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004901
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A polyphasic taxonomic approach was used to characterize a Gram-stain-negative bacterium, designated strain CC-YST696 T, harbouring antibiotic- and toxic compound-resistace genes, isolated from poultry manure in Taiwan. Cells of CC-YST696 T were short rods, motile with polar flagella, catalase- and oxidase-positive. Optimal growth occurred at 30 °С, pH 9 and with 1 % NaCl. The results of phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by CC-YST696 T associated with Devosia chinhatensis (97.9 % sequence identity), Devosia riboflavina (97.3 %) and Devosia indica (97.2 %), and with lower sequence similarity values to other species. Average nucleotide identity (ANI) values were 72.8–80.0 % ( n =17) compared within the type strains of species of of the genus Devosia . CC-YST696 T contained C16:0, C18:0, C18:1 ω7 c 11-methyl and C18:1 ω6 c / C18:1 ω7 c as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, three unidentified glycolipids, two unidentified phospholipids and three unidentified lipids. The DNA G+C content was 62.2 mol% and the predominant quinone was ubiquinone Q-10. On the basis of its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-YST696 T is proposed to represent a novel species of the genus Devosia, for which the name Devosia faecipullorum sp. nov. (type strain CC-YST696 T =BCRC 81284 T =JCM 34167 T ) is proposed.