A novel UV-curable extravascular stent to prevent restenosis of venous grafts. (15th November 2021)
- Record Type:
- Journal Article
- Title:
- A novel UV-curable extravascular stent to prevent restenosis of venous grafts. (15th November 2021)
- Main Title:
- A novel UV-curable extravascular stent to prevent restenosis of venous grafts
- Authors:
- Dai, Chun
Chu, Tianshu
Li, Xiang
Jiang, Hui
Liu, Tan
Zhou, Yang
Gao, Lei
Shen, Cheng
Ge, Jianjun - Abstract:
- Abstract: The incidence of coronary heart disease is increasing yearly. Coronary artery bypass graft (CABG) is an important surgical method for the treatment of coronary heart disease. The vein grafts in CABG surgery mostly use saphenous vein, but restenosis of the graft frequently occurs, and has become an urgent clinical problem. In this study, we produced a UV-curable Hydroxyethyl Methacrylate (HEMA) gel containing Bletilla striata, and compared its tensile strength and expansibility to that of human vessels, showing that the film has good physical properties. Through cell experiments, we proved that film has no effect on cell proliferation, migration, apoptosis, and DNA damage. In addition, we placed the film under the skin of mice to measure inflammation-related cells and factors, and found that it did not trigger the body's immune response. After that, we designed a rat vein graft model to simulate the pathological changes of the vein graft vessel after CABG. The film had a good inhibitory effect on the restenosis of vein graft vessels in the rat model. Finally, analysis of the expression levels of proteins involved in the proliferation and migration indicated that the gel can effectively inhibit these processes in smooth muscle cells in the graft vein. In conclusion, this study has successfully produced a UV-curable HEMA gel containing B. striata that could effectively inhibit the restenosis of vein grafts. Graphical abstract: Fig Abstract: a: In CABG, the saphenousAbstract: The incidence of coronary heart disease is increasing yearly. Coronary artery bypass graft (CABG) is an important surgical method for the treatment of coronary heart disease. The vein grafts in CABG surgery mostly use saphenous vein, but restenosis of the graft frequently occurs, and has become an urgent clinical problem. In this study, we produced a UV-curable Hydroxyethyl Methacrylate (HEMA) gel containing Bletilla striata, and compared its tensile strength and expansibility to that of human vessels, showing that the film has good physical properties. Through cell experiments, we proved that film has no effect on cell proliferation, migration, apoptosis, and DNA damage. In addition, we placed the film under the skin of mice to measure inflammation-related cells and factors, and found that it did not trigger the body's immune response. After that, we designed a rat vein graft model to simulate the pathological changes of the vein graft vessel after CABG. The film had a good inhibitory effect on the restenosis of vein graft vessels in the rat model. Finally, analysis of the expression levels of proteins involved in the proliferation and migration indicated that the gel can effectively inhibit these processes in smooth muscle cells in the graft vein. In conclusion, this study has successfully produced a UV-curable HEMA gel containing B. striata that could effectively inhibit the restenosis of vein grafts. Graphical abstract: Fig Abstract: a: In CABG, the saphenous vein is removed and grafted onto the coronary arteries of the heart. b:The gelatinous scaffold is wrapped around the vessel while in liquid state. After UV curing, the scaffold can provide support for the vein graft. Image 1 Highlights: The extravascular stents can be cured under UV light. The extravascular stent can encapsulate Bletilla striata polysaccharide and release it stably. The extravascular stents can effectively inhibit vascular restenosis. The extravascular stent has good histocompatibility and cytocompatibility. … (more)
- Is Part Of:
- Composites. Number 225(2021)
- Journal:
- Composites
- Issue:
- Number 225(2021)
- Issue Display:
- Volume 225, Issue 225 (2021)
- Year:
- 2021
- Volume:
- 225
- Issue:
- 225
- Issue Sort Value:
- 2021-0225-0225-0000
- Page Start:
- Page End:
- Publication Date:
- 2021-11-15
- Subjects:
- Hydroxyethyl methacrylate -- Bletilla striata polysaccharide -- Coronary artery bypass grafting -- Intimal hyperplasia -- Restenosis
CABG coronary artery bypass grafting -- CCK8 cell proliferation assay -- DA dopamine hydrochloride -- DDS drug delivery systems -- DMA dynamic mechanical analysis -- DMA dynamic thermomechanical analysis -- FITC fluorescein isothiocyanate -- FTIR fourier transform infrared -- HEMA hydroxyethyl methacrylate -- HRP horseradish peroxidase -- HUVECs human umbilical vein endothelial cells -- IH intimal hyperplasia -- PCL polycaprolactone -- PI propidium iodide -- PTFE polytetrafluoroethylene -- SCGE single cell gel electrophoresis -- SEM scanning electron microscope -- SSNMR solid state nuclear magnetic resonance -- TGA thermogravimetric analysis
Composite materials -- Periodicals
Materials science -- Periodicals
Composite materials
Periodicals
Electronic journals
620.118 - Journal URLs:
- http://www.sciencedirect.com/science/journal/13598368 ↗
http://www.elsevier.com/journals ↗ - DOI:
- 10.1016/j.compositesb.2021.109260 ↗
- Languages:
- English
- ISSNs:
- 1359-8368
- Deposit Type:
- Legaldeposit
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- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3365.620000
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