Biologically synthesized silver nanoparticles by Streptomyces sp. EMB24 extracts used against the drug-resistant bacteria. (September 2021) - Goel, Nikky
Abstract: Drug resistance to common antibiotics has resurfaced, prompting the development of novel therapeutics such as nanoparticles (NPs). In this work, the silver nanoparticles (AgNPs) were synthesized by the cell-free extract produced by the Streptomyces strain and characterized using standard analytical techniques. The size of NPs was found to be in the range of 10-30 nm. The biologically synthesized AgNPs exhibit potent antibacterial activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. In addition, AgNPs inhibited the growth of clinically isolated pathogenic strains, including Methicillin-resistant Staphylococcus aureus (MRSA) and tetracycline-resistant Neisseria gonorrhoeae . Furthermore, these AgNPs have reduced the development of Pseudomonas aeruginosa biofilm by 80%, one of the key contributors to the increased resistance among bacteria. The inhibition was confirmed using crystal violet assay and confocal microscopic images indicating the future application of AgNPs as a coating material on medical devices used in treating patients to inhibit biofilm formation. Graphical abstract: Unlabelled Image Highlights: Antimicrobial resistance prompting the development of novel therapeutics Silver nanoparticles inhibited drug-resistant bacteria. Silver nanoparticles inhibited formation of biofilm. FESEM images revealed the size range was 10–30 nm.