A guide to the design of synthetic gene networks in mammalian cells. (14th December 2020)

• Record Type: Journal Article
• Authors: Verbič, Anže; Praznik, Arne; Jerala, Roman
• Is Part Of: FEBS journal. Volume 288:Number 18(2021)
• Page Start: 5265
• Page End: 5288
• Publication Date: 2020-12-14
• Subjects: Boolean logic -- gene networks -- signal processing -- synthetic biology; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.15652
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : Synthetic biology aims to harness natural and synthetic biological parts and engineering them in new combinations and systems, producing novel therapies, diagnostics, bioproduction systems, and providing information on the mechanism of function of biological systems. Engineering cell function requires the rewiring or de novo construction of cell information processing networks. Using natural and synthetic signal processing elements, researchers have demonstrated a wide array of signal sensing, processing and propagation modules, using transcription, translation, or post‐translational modification to program new function. The toolbox for synthetic network design is ever‐advancing and has still ample room to grow. Here, we review the diversity of synthetic gene networks, types of building modules, techniques of regulation, and their applications. Abstract : Synthetic circuits have evolved from simple switches, to complex interwoven cellular networks, which are capable of precisely processing desired input signals and regulating an output in a predictable, tunable manner. The goal of such systems is to allow researchers to exert unprecedented control over cellular function, by either coupling the circuits with innate cellular mechanisms or introducing novel functions. This advancement brings important implications in several biological applications.