An updated classification and mechanistic insights into ligand binding of the substrate‐binding proteins. Issue 18 (20th August 2021)

• Record Type: Journal Article
• Authors: Chandravanshi, Monika; Tripathi, Sisir Kant; Kanaujia, Shankar Prasad
• Is Part Of: FEBS letters. Volume 595:Issue 18(2021)
• Page Start: 2395
• Page End: 2409
• Publication Date: 2021-08-20
• Subjects: binding protein -- domain movement -- receptors -- structure‐based classification -- subdomains -- 'venus fly‐trap' mechanism; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.14174
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Substrate‐binding proteins (SBPs) mediate ligand translocation and have been classified into seven clusters (A‐G). Although the substrate specificities of these clusters are known to some extent, their ligand‐binding mechanism(s) remain(s) incompletely understood. In this study, the list of SBPs belonging to different clusters was updated (764 SBPs) compared to the previously reported study (504 SBPs). Furthermore, a new cluster referred to as cluster H was identified. Results reveal that SBPs follow different ligand‐binding mechanisms. Intriguingly, the majority of the SBPs follow the 'one domain movement' rather than the well‐known 'Venus Fly‐trap' mechanism. Moreover, SBPs of a few clusters display subdomain conformational movement rather than the complete movement of the N‐ and C‐terminal domains. Abstract :