Blocking of interleukin-1 suppresses angiotensin II-induced renal injury. Issue 17 (17th September 2021)

• Record Type: Journal Article
• Authors: Akita, Koji; Isoda, Kikuo; Ohtomo, Fumie; Isobe, Sarasa; Niida, Tomiharu; Sato-Okabayashi, Yayoi; Sano, Motoaki; Shimada, Kazunori; Iwakura, Yoichiro; Minamino, Tohru
• Is Part Of: Clinical science. Volume 135:Issue 17(2021)
• Page Start: 2035
• Page End: 2048
• Publication Date: 2021-09-17
• Subjects: chronic kidney disease -- inflammation -- interleukin-1 -- renin-angiotensin system; Medicine -- Periodicals; Biochemistry -- Periodicals; 616
• DOI: 10.1042/CS20201406
• Languages: English
• ISSNs: 0143-5221

Abstract

Abstract: Clinical hypertension (HT) is associated with renal inflammation and elevated circulating levels of proinflammatory cytokines. Interleukin (IL)-1 receptor antagonist (IL-1Ra) is one of the most important anti-inflammatory cytokines and plays a crucial role in inflammation. Inhibition of IL-1 may contribute to modulation of the Angiotensin II (Ang II)-induced HT response. The present study aimed to elucidate the effects of IL-1Ra and anti-IL-1β antibody (01BSUR) on Ang II-induced renal injury. To determine the contribution of IL-1Ra to Ang II-induced renal inflammation, male wildtype (WT) and IL-1Ra-deficient (IL-1Ra −/− ) mice were infused with Ang II (1000 ng/kg/min) using subcutaneous osmotic pump for 14 days. We checked renal function, histological change, and several mRNA expressions 14 days after infusion. Fourteen days after infusion, systolic blood pressure (197 ± 5 vs 169 ± 9 mmHg, P <0.05) in IL-1Ra −/− mice significantly increased compared with WT mice. Furthermore, on day 14 of Ang II infusion, plasma IL-6 was 5.9-fold higher in IL-1Ra −/− versus WT mice ( P <0.001); renal preproendothelin-1 mRNA expression was also significantly higher in IL-1Ra −/− mice ( P <0.05). In addition, renal histology revealed greater damage in IL-1Ra −/− mice compared with WT mice 14 days after infusion. Finally, we administrated 01BSUR to both IL-1Ra −/− and WT mice, and 01BSUR treatment decreased Ang II-induced HT and renal damage (glomerular injury and fibrosis of the tubulointerstitial area) in both IL-1Ra −/− and WT mice compared with IgG2a treatment. Inhibition of IL-1 decreased Ang II-induced HT and renal damage in both IL-1Ra −/− and WT mice, suggesting suppression of IL-1 may provide an additional strategy to protect against renal damage in hypertensive patients.