Vagotomy and Splenectomy Reduce Insulin Secretion and Interleukin-1β. Issue 4 (April 2021)

• Record Type: Journal Article
• Authors: Siqueira, Bruna Schumaker; Ceglarek, Vanessa Marieli; Gomes, Ellen Carolina Zawoski; Vettorazzi, Jean Franciesco; Rentz, Thiago; Nenevê, Juliane Zanon; Volinski, Karoline Zanella; Moraes, Sandra Schmidt; Malta, Ananda; de Freitas Mathias, Paulo Cezar; de Oliveira Emilio, Henriette Rosa; Balbo, Sandra Lucinei; Grassiolli, Sabrina
• Is Part Of: Pancreas. Volume 50:Issue 4(2021)
• Publication Date: 2021-04
• Subjects: autonomic nervous system -- spleen -- insulin -- pancreatic islets; Pancreas -- Diseases -- Periodicals; Pancreas -- Periodicals; Neuroendocrine tumors -- Periodicals; 616.37005
• DOI: 10.1097/MPA.0000000000001809
• Languages: English
• ISSNs: 0885-3177

Abstract

Abstract : Objectives: This study aimed to evaluate the effect of vagotomy, when associated with splenectomy, on adiposity and glucose homeostasis in Wistar rats. Methods: Rats were divided into 4 groups: vagotomized (VAG), splenectomized (SPL), VAG + SPL, and SHAM. Glucose tolerance tests were performed, and physical and biochemical parameters evaluated. Glucose-induced insulin secretion and protein expression (Glut2/glucokinase) were measured in isolated pancreatic islets. Pancreases were submitted to histological and immunohistochemical analyses, and vagus nerve neural activity was recorded. Results: The vagotomized group presented with reduced body weight, growth, and adiposity; high food intake; reduced plasma glucose and triglyceride levels; and insulin resistance. The association of SPL with the VAG surgery attenuated, or abolished, the effects of VAG and reduced glucose-induced insulin secretion and interleukin-1β area in β cells, in addition to lowering vagal activity. Conclusions: The absence of the spleen attenuated or blocked the effects of VAG on adiposity, triglycerides and glucose homeostasis, suggesting a synergistic effect of both on metabolism. The vagus nerve and spleen modulate the presence of interleukin-1β in β cells, possibly because of the reduction of glucose-induced insulin secretion, indicating a bidirectional flow between autonomous neural firing and the spleen, with repercussions for the endocrine pancreas.