Jujube polysaccharides mitigated anemia in rats with chronic kidney disease: Regulation of short chain fatty acids release and erythropoietin production. (November 2021)

• Record Type: Journal Article
• Authors: Huang, Shiying; Jiang, Xue; Chen, Qiugu; Hu, Zhaoliu; Wang, Fochang; Zhao, Yuan; Xin, Gui-Zhong; Chen, Jianping
• Is Part Of: Journal of functional foods. Volume 86(2021)
• Publication Date: 2021-11
• Subjects: Jujube polysaccharides -- Chronic kidney disease -- Anaemia -- Erythropoietin -- Hypoxia inducible factor-α -- Short chain fatty acids; AA acetic acid -- BA butyric acid -- BUN blood urea nitrogen -- CKD chronic kidney disease -- GC–MS gas chromatography mass spectrometry -- EPO erythropoietin -- HA hexanoic acid -- Hb hemoglobin -- HCT hematocrit -- HIF-1α hypoxia inducible factor-1α -- HQC high concentration quality control samples -- HRP horseradish peroxidase -- IBA isobutyric acid -- ISTD internal standard -- IVA isovaleric acid -- JP jujube polysaccharides -- LQC low concentration quality control samples -- MQC medium concentration quality control samples -- MRM multiple-reaction monitoring -- PA propanoic acid -- PAS Periodic acid-Schiff -- PLT platelet count -- RBC red blood cell -- SCFAs short chain fatty acids -- Scr serum creatinine -- SIM selected ion monitoring -- UHPLC-MS/MS ultra high performance liquid chromatography tandem mass spectrometry -- VA valeric acid -- 2-BA 2-methylbutyric acid; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104673
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Jujube polysaccharides stimulates EPO expression via HIF-α accumulation. Jujube polysaccharides treatment improves kidney function and anemia in CKD rats. Jujube polysaccharides significantly increases the release of SCFAs in CKD rats. A derivatization-based LC-MS method is developed for SCFAs determination in feces. Abstract: Jujube polysaccharides (JP) is one of the active food sourced glycans in dietary fruit of Ziziphus jujuba, which has been considered for the treatment of blood deficiency. In this study, a chronic kidney disease (CKD) rat model was used to evaluate the effect of JP and its mechanism on CKD-associated anemia. In CKD rats, JP treatment significantly ameliorated kidney function, kidney pathological injury and hematological parameters, including increasing red blood cells, hemoglobin, hematocrit and platelet count. Moreover, LC-MS/MS targeted metabolomics results showed that JP promoted short chain fatty acids (SCFAs) release in CKD rats. Besides, JP alternated the serum erythropoietin (EPO) level, kidney EPO mRNA and kidney EPO protein via HIF-α signaling. Collectively, these results provide evidence that the effect of JP on CKD-associated anemia may be involved in regulation of SCFAs release and erythropoietin production, supporting further development of JP as food supplements in treating CKD-associated anemia.