Optimization of E. coli tip‐sonication for high‐yield cell‐free extract using finite element modeling. Issue 10 (12th August 2021)

• Record Type: Journal Article
• Authors: Ferdous, Sakib; Dopp, Jared L.; Reuel, Nigel F.
• Is Part Of: AIChE journal. Volume 67:Issue 10(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-08-12
• Subjects: bacterial cell lysis -- cell‐free protein synthesis -- protein‐denaturation -- sonolysis -- tip‐sonication -- ultrasonic cell disruption; Chemical engineering -- Periodicals; Génie chimique -- Périodiques; 660.28
• DOI: 10.1002/aic.17389
• Languages: English
• ISSNs: 0001-1541

Abstract

Abstract: Optimal tip sonication settings, namely tip position, input power, and pulse durations, are necessary for temperature sensitive procedures like preparation of viable cell extract. In this paper, the optimum tip immersion depth (20–30% height below the liquid surface) is estimated which ensures maximum mixing thereby enhancing thermal dissipation of local cavitation hotspots. A finite element (FE) heat transfer model is presented, validated experimentally with ( R 2 > 97%) and used to observe the effect of temperature rise on cell extract performance of Escherichia coli BL21 DE3 star strain and estimate the temperature threshold. Relative yields in the top 10% are observed for solution temperatures maintained below 32°C; this reduces below 50% relative yield at temperatures above 47°C. A generalized workflow for direct simulation using the CONSOL code as well as master plots for estimation of sonication parameters (power input and pulse settings) is also presented.