In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens. Issue 41 (1st October 2021)

• Record Type: Journal Article
• Authors: Fujisawa, Sotaro; Murata, Shiro; Takehara, Masaki; Aoyama, Julia; Morita, Ayu; Isezaki, Masayoshi; Win, Shwe Yee; Ariizumi, Takuma; Sato, Takumi; Oishi, Eiji; Taneno, Akira; Maekawa, Naoya; Okagawa, Tomohiro; Ichii, Osamu; Konnai, Satoru; Ohashi, Kazuhiko
• Is Part Of: Vaccine. Volume 39:Issue 41(2021)
• Page Start: 6057
• Page End: 6066
• Publication Date: 2021-10-01
• Subjects: Poultry red mite -- Adipocyte plasma membrane-associated protein -- Vaccine -- Dg-APMAP -- Dermanyssus gallinae; PRM poultry red mite -- APMAP adipocyte plasma membrane-associated protein -- PBS phosphate-buffered saline -- RNA-Seq RNA-sequencing -- ORF open reading frame -- LCM laser-capture microdissection -- RT-PCR reverse transcription polymerase chain reaction -- Elf1a1 elongation factor 1-α 1-like gene -- qPCR quantitative PCR -- SDS sodium dodecyl sulfate -- ELISA enzyme-linked immunosorbent assay -- PBS-T PBS containing 0.05% Tween 20 -- SDS-PAGE SDS-polyacrylamide gel electrophoresis; Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2021.08.104
• Languages: English
• ISSNs: 0264-410X

Abstract

Highlights: Infestation with PRMs causes economic losses in the poultry industry. Vaccination has been studied as an alternative method for controlling PRMs. We identified a novel APMAP-like molecule in PRMs (Dg-APMAP). Mortality was increased in PRMs fed with immune plasma. Vaccination targeting Dg-APMAP could be an effective method for PRM control. Abstract: The poultry red mite ( Dermanyssus gallinae ; PRM) is a blood-sucking ectoparasite of chickens that is a threat to poultry farming worldwide and significantly reduces productivity in the egg-laying industry. Chemical acaricides that are widely used in poultry farms for the prevention of PRMs are frequently ineffective due to the emergence of acaricide-resistant PRMs. Therefore, alternative control methods are needed, and vaccination is a promising strategy for controlling PRMs. A novel adipocyte-plasma membrane-associated protein-like molecule (Dg-APMAP) is highly expressed in blood-fed PRMs according to a previous RNA sequencing analysis. Here, we attempted to identify the full sequence of Dg-APMAP, study its expression in different life stages of PRMs, and evaluate its potential as a vaccine antigen. Dg-APMAP mRNA was expressed in the midgut and ovaries, and in all life stages regardless of feeding states. Importantly, in vitro feeding of PRMs with plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-APMAP significantly reduced their survival rate in nymphs and adults, which require blood meals. Our data suggest that the host immune responses induced by vaccination with Dg-APMAP could be an effective strategy to reduce the suffering caused by PRMs in the poultry industry.