Alginate bone scaffolds coated with a bioactive lactose modified chitosan for human dental pulp stem cells proliferation and differentiation. (1st December 2021)

• Record Type: Journal Article
• Authors: Porrelli, Davide; Gruppuso, Martina; Vecchies, Federica; Marsich, Eleonora; Turco, Gianluca
• Is Part Of: Carbohydrate polymers. Volume 273(2021)
• Publication Date: 2021-12-01
• Subjects: ALP alkaline phosphatase -- BMSCs bone marrow stromal cells -- CTL lactose-modified chitosan -- CTL-sc alginate/hydroxyapatite scaffolds coated with CTL -- Ctrl-sc alginate/hydroxyapatite scaffolds -- DS differentiation stimuli -- hDPSCs human dental pulp stem cells -- MSCs mesenchymal stem cells -- SBF Simulated Body Fluid; Scaffolds -- Alginate -- Bioactive lactose-modified chitosan -- Human dental pulp stem cells -- Bioactive polysaccharides -- Bone regeneration; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2021.118610
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Bioactive and biodegradable porous scaffolds can hasten the healing of bone defects; moreover, patient stem cells seeded onto scaffolds can enhance the osteoinductive and osteoconductive properties of these biomaterials. In this work, porous alginate/hydroxyapatite scaffolds were functionalized with a bioactive coating of a lactose-modified chitosan (CTL). The highly interconnected porous structure of the scaffold was homogeneously coated with CTL. The scaffolds showed remarkable stability up to 60 days of aging. Human Dental Pulp Stem Cells (hDPSCs) cultured in the presence of CTL diluted in culture medium, showed a slight and negligible increase in terms of proliferation rate; on the contrary, an effect on osteogenic differentiation of the cells was observed as a significant increase in alkaline phosphatase activity. hDPSCs showed higher cell adhesion on CTL-coated scaffolds than on uncoated ones. CTL coating did not affect cell proliferation, but stimulated cell differentiation as shown by alkaline phosphatase activity analysis. Highlights: Lactose modified chitosan (CTL) stimulates dental pulp stem cells differentiation. Freeze-drying was used to prepare alginate/hydroxyapatite porous scaffolds. CTL can be used as a bioactive coating of alginate/hydroxyapatite bone scaffolds. CTL coating of scaffolds positively affects cell adhesion and scaffold colonization.