Highly active biological dermal acellular tissue scaffold composite with human bone powder for bone regeneration. (1st November 2021)

• Record Type: Journal Article
• Authors: Sun, Yang; Li, Ruixue; Yu, Xiaohua; Li, Xueyan; Han, Zhihui; Sun, Jian; Bi, Wei; Liu, Wenjuan; Yu, Youcheng; Cui, Wenguo
• Is Part Of: Materials & design. Volume 209(2021)
• Publication Date: 2021-11-01
• Subjects: Bp/ATM composite scaffold -- Biological macromolecules -- Active osteoinductive -- Bone regeneration; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2021.109963
• Languages: English
• ISSNs: 0264-1275

Abstract

Graphical abstract: Sodium deoxycholate is used to decellularize porcine dermis and obtain ATM with low immunogenicity. The ATM and human-derived Bp are mixed in different volume ratios to prepare a highly active biomacromolecule scaffold with osteoinductive and osteoconductive activities in a simulated bone healing microenvironment. A rat cranial defect model was used to evaluate the effects of different scaffolds on bone regeneration. Highlights: Sodium deoxycholate decellularizes porcine dermis to obtain acellular tissue matrix (ATM) with low immunogenicity. ATM combines with different contents of human bone powder (Bp). Highly active biomacromolecule three-dimensional scaffold with osteoinductive and osteoconductive properties. Bp/ATM perfectly simulate the natural osteogenesis microenvironment. The 50 %Bp/ATM scaffold promotes bone regeneration in rat cranial defects. Abstract: Regenerative medicine for bone tissue in the oral cavity aims to repair alveolar or jaw defects. An ideal, highly active, osteoinductive, three-dimensional scaffold that can effectively promote cell proliferation and differentiation has not been reported. This study evaluated the in vitro and in vivo efficacy of a biological macromolecule composite scaffold derived from porcine dermal matrix bone tissue decellularized using sodium deoxycholate technology. The composite scaffold was composed of a porcine dermal acellular tissue matrix and human bone powder. The bone powder/acellular tissue matrix (Bp/ATM) scaffold was porous with a porosity exceeding 70%. Bp/ATM provided an osteoinductive scaffold that recruited osteoblasts to the site of injury and promoted their differentiation to bone cells. The 50% (v/v) bone-scaffold mixture significantly accelerated mineralized matrix production and the expression of osteogenesis-related proteins during osteogenic differentiation in vitro . This mixture also enhanced bone regeneration at the defect site in vivo in a rat cranial defect model. These observations suggest that Bp/ATM forms a highly active osteoinductive and osteoconductive scaffold that is a promising biomaterial for bone regeneration.