Gastroprotective effects and metabolomic profiling of Chasteberry fruits against indomethacin-induced gastric injury in rats. (November 2021)

• Record Type: Journal Article
• Authors: Ogaly, Hanan A.; Alsherbiny, Muhammad A.; El Badawy, Shymaa A.; Abd-Elsalam, Reham M.; Guang Li, Chun; Azouz, Asmaa A.
• Is Part Of: Journal of functional foods. Volume 86(2021)
• Publication Date: 2021-11
• Subjects: Indomethacin -- Gastric injury -- Vitex agnus castus -- Apoptosis -- NF-κB signalling -- Metabolomic profiling -- UPLC-QTOF-MS; COX-2 Cycloxygenase-2 -- GSH Reduced glutathione -- HSP-70 Heat shock protein 70 kda -- IND indomethacin -- MDA Malonaldehyde -- MPO Myeloperoxidase -- NF-κB1 nuclear factor kappa-B, subunite 1 -- NSAID Nonsteroidal anti-inflammatory drugs -- PGE2 prostaglandin E2 -- SOD Superoxide dismutase -- TNF-α Tumour necrosis factor-alpha -- VACE Vitex agnus castus L. extract; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2021.104732
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: This study is the first to highlight the potential gastro-protective activity of Vitex agnus castus extract VACE, the effect may be related to its arsenal metabolites. VACE (120 mg/kg) showed a comparable protection as esomeprazole in gastroprotection scoring, antioxidative and anti-apoptotic activity-related parameters. Antioxidant, anti-inflammatory, and regulation of NF-κß and apoptosis pathways are among the mechanisms of VACE-induced protective activity. This beneficial effects of VACE suggested it as a promising natural anti-ulcerogenic supplement. Abstract: Vitex agnus castus L. extract (VACE) was investigated for its gastroprotective properties and possible molecular mechanisms in rats. VACE (60 or 120 mg/kg) or Esomeprazole (20 mg/kg) were orally administered for 3 weeks before the induction of gastropathy using indomethacin (30 mg/kg, single oral dose). VACE ameliorated the indomethacin-induced gastric juice acidity and pathological changes. VACE significantly preserved GSH, SOD, NO and PGE2 contents, while decreased lipid-peroxide, TNF-α and MPO contents. Moreover, VACE downregulated NF-κB1, COX-2, Caspase-3 and upregulated Bcl-2 and HSP-70 expression. Ultra-high-performance liquid chromatography (UPLC) coupled with quadrupole high-resolution time of flight mass spectrometry (qTOF-MS) enabled the tentative identification of 87 compounds allocated in seven main classes including flavonoids, glycosylated iridoid and labdane diterpenes. Notably, different agnuside derivatives and diterpenoids were reported in VACE for the first time. In conclusion, VACE contains an arsenal of bioactive metabolites which may exhibit gastroprotection by inhibiting inflammation, oxidative stress, and apoptosis.