THU0328 A Comprehensive Evaluation of Serum Autoantibodies in Primary Sjögren's Syndrome. (15th July 2016)

• Record Type: Journal Article
• Authors: Capaldo, C.; Carvajal Alegria, G.; Bendaoud, B.; Jousse-Joulin, S.; Saraux, A.; Pers, J.-O.; Devauchelle-Pensec, V.; Renaudineau, Y.
• Is Part Of: Annals of the rheumatic diseases. Volume 75(2016)Supplement 2
• Page Start: 306
• Page End: 306
• Publication Date: 2016-07-15
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/annrheumdis-2016-eular.3392
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : Background: Conventional antibodies are well described and studied in primary Sjögren syndrome, nevertheless patients can also present unconventional autoantibodies. Objectives: The objective of this study was to investigate the prevalence of a large panel of conventional and unconventional autoantibodies (Ab) in a well-characterized population of patients with primary Sjögren's syndrome (pSS). Methods: Two hundred and twenty six pSS patients benefiting from a comprehensive clinical and radiological assessment were recruited. A new technique based on chemiluminescence (ISYS-IDS) was selected, in 44 patients, to assay 17 autoantigens including SSA/Ro (52+60 kDa), SSB/La, dsDNA, centromere, Sm, U1-snRNP, Scl70, Jo1, cardiolipin, β2-GPI, CCP2, PR3, MPO, GBM, transglutaminase (IgA), and deaminated gliadin (IgA&IgG). If positive, the presence of Ab was validated by another technique. The study was completed by the search for anti-nuclear Ab (ANA) on HEp-2 cells and rheumatoid factors (RF) against human and rabbit IgG. Results: Search for conventional Ab showed ANA (71.7% at 1:320), anti-SSA/Ro Ab (61.1%), anti-SSB/La Ab (31.0%, all anti-SSA+) and IgM RF (44.2%). The triple Ab association (ANA+SSA+RF) rather than the SSA/SSB association was associated with an earlier diagnosis, an active immunological profile (hypergammaglobulinemia, erythrocyte sedimentation rate) and a salivary gland involvement (lymphocyte infiltration, ultrasonography and enlargement). Three patients out of 44 (6.8%) had unintended Ab in current practice directed against centromere, Jo1, and dsDNA plus IgG deaminated gliadin Ab but without associated clinical signs. Conclusions: The ability to combine a wide range of autoantibodies can lead to defining new subgroups of pSS patients for precise disease characterization, patient stratification and response prediction. Disclosure of Interest: None declared