FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco. (November 2006)

• Record Type: Journal Article
• Authors: Estévez, José M.; Somerville, Chris
• Is Part Of: Biotechniques. Volume 41:Number 5(2006)
• Page Start: 569
• Page End: 574
• Publication Date: 2006-11
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000112264
• Languages: English
• ISSNs: 0736-6205

Abstract

Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter. Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.