Optimization of HIV-1 infectivity assays. (November 2007)

• Record Type: Journal Article
• Authors: Jones, Jennifer; Whitford, William; Wagner, Frederic; Kutsch, Olaf
• Is Part Of: Biotechniques. Volume 43:Number 5(2007)
• Page Start: 589
• Page End: 594
• Publication Date: 2007-11
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000112624
• Languages: English
• ISSNs: 0736-6205

Abstract

HIV-1 reporter cell lines are the backbone of diagnostic assays, vaccine and drug development efforts. Performing HIV-1 infection experiments in a T cell background is desirable for many reasons. However, a low susceptibility to infection with primary patient isolates in available reporter T cell lines has limited such efforts. We here demonstrate that optimization of HIV-1 receptor expression and the utilization of serum free medium compositions can increase susceptibility of reporter T cell lines to HIV-1 infection by up to two orders of magnitude.