Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay. (March 2008)

• Record Type: Journal Article
• Authors: Abramovitz, Mark; Ordanic-Kodani, Maja; Wang, Yuefang; Li, Zhenhong; Catzavelos, Charles; Bouzyk, Mark; Sledge, George W.; Moreno, Carlos S.; Leyland-Jones, Brian
• Is Part Of: Biotechniques. Volume 44:Number 3(2008)
• Page Start: 417
• Page End: 423
• Publication Date: 2008-03
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000112703
• Languages: English
• ISSNs: 0736-6205

Abstract

Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result in the preparation of total RNA that is of the necessary quality for use in the DASL (cDNA-mediated annealing, selection, extension, and ligation) assay in which expression of 502 genes are analyzed simultaneously using as little as 100 ng of input RNA.