Whole-mount in situ hybridization of small invertebrate embryos using laboratory mini-columns. (December 2007)

• Record Type: Journal Article
• Authors: Irvine, Steven Q.
• Is Part Of: Biotechniques. Volume 43:Number 6(2007)
• Page Start: 764
• Page End: 768
• Publication Date: 2007-12
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000112617
• Languages: English
• ISSNs: 0736-6205

Abstract

To facilitate the handling of small invertebrate embryos during whole-mount in situ hybridization, a method of solution exchange using laboratory mini-columns was developed. This protocol speeds time-consuming aspiration of buffers, and avoids accidental loss of the embryos, by gently pushing solutions through the column using air pressure from a syringe after each incubation. The next buffer is then added using a pipettor. Embryos are retained on a filter within the column. As many columns as desired may be processed in parallel for different probes or stages.