Quantification of small cell numbers with a microchannel device. (September 2008)

• Record Type: Journal Article
• Authors: Badders, Nisha M.; Yu, Hongmei; Alexander, Caroline M.; Beebe, David J.
• Is Part Of: Biotechniques. Volume 45:Number 3(2008)
• Page Start: 321
• Page End: 325
• Publication Date: 2008-09
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000112906
• Languages: English
• ISSNs: 0736-6205

Abstract

The ability to precisely quantify rare populations of cells has become an essential first step of many cell-based assays in stem cell research. Since current devices for cell quantification require relatively high cell concentrations and/or absolute cell numbers, we have developed a microchannel-based device, allowing precise quantification of limiting cell numbers/concentrations. We anticipate this device will serve as an important tool to overcome a practical obstacle in stem cell research.