Coupled rolling circle amplification loop-mediated amplification for rapid detection of short DNA sequences. (September 2008)

• Record Type: Journal Article
• Authors: Marciniak, Jennifer Y.; Kummel, Andrew C.; Esener, Sadik C.; Heller, Michael J.; Messmer, Bradley T.
• Is Part Of: Biotechniques. Volume 45:Number 3(2008)
• Page Start: 275
• Page End: 280
• Publication Date: 2008-09
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000112910
• Languages: English
• ISSNs: 0736-6205

Abstract

Circularizable oligonucleotide probes can detect short DNA sequences with single-base resolution at the site of ligation and can be amplified by rolling circle amplification (RCA) using strand displacing polymerases. A secondary amplification scheme was developed that uses the loop-mediated amplification reaction concurrent with RCA to achieve rapid signal development from the starting circular molecules. This isothermal reaction was found to be significantly faster than the comparable hyperbranching amplification method and could detect 100 circular copies in less than 1 h.