Mass spectrometry provides accurate characterization of two genetic marker types in Bacillus anthracis. (October 2004)

• Record Type: Journal Article
• Authors: Ert, Matthew N. Van; Hofstadler, Steven A.; Jiang, Yun; Busch, Joseph D.; Wagner, David M.; Drader, Jared J.; Ecker, David J.; Hannis, James C.; Huynh, Lynn Y.; Schupp, James M.; Simonson, Tatum S.; Keim, Paul
• Is Part Of: Biotechniques. Volume 37:Number 4(2004)
• Page Start: 642
• Page End: 651
• Publication Date: 2004-10
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/04374RR01
• Languages: English
• ISSNs: 0736-6205

Abstract

Epidemiological and forensic analyses of bioterrorism events involving Bacillus anthracis could be improved if both variable number tandem repeats (VNTRs) and single nucleotide polymorphisms (SNPs) could be combined on a single analysis platform. Here we present the use of electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS) to characterize 24 alleles from 6 VNTR loci and 11 alleles from 7 SNP loci in B. anthracis . The results obtained with ESI-FTICRMS were consistent with independent results obtained from traditional approaches using electrophoretic detection of fluorescent products. However, ESI-FTICR-MS improves on the traditional approaches because it does not require fluorescent labeling of PCR products, minimizes post-PCR processing, obviates electrophoresis, and provides unambiguous base composition of both SNP and VNTR PCR products. In addition, ESI-FTICR-MS allows both marker types to be examined simultaneously and at a rate of approximately 1 sample per min. This technology represents a significant advance in our ability to rapidly characterize B. anthracis isolates using VNTR and SNP loci.