Comparison of different probe-level analysis techniques for oligonucleotide microarrays. (February 2004)

• Record Type: Journal Article
• Authors: Rosati, Barbara; Grau, Frederic; Kuehler, Anneke; Rodriguez, Samantha; McKinnon, David
• Is Part Of: Biotechniques. Volume 36:Number 2(2004)
• Page Start: 316
• Page End: 322
• Publication Date: 2004-02
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/04362MT03
• Languages: English
• ISSNs: 0736-6205

Abstract

Three different software packages for the probe-level analysis of high-density oligonucleotide microarray data were compared using an experiment-derived data set that was validated using real-time PCR. The efficiency with which these three programs could identify true positives in this data set was assessed. In addition, estimates of false-positive and false-negative rates were determined. The performance of the programs using very small data sets was also compared, and recommendations for use are suggested.