A simplified, 96-well–adapted, ATP luminescence–based motility assay. (October 2009)

• Record Type: Journal Article
• Authors: Restouin, Audrey; Aresta, Sandra; Prébet, Thomas; Borg, Jean-Paul; Badache, Ali; Collette, Yves
• Is Part Of: Biotechniques. Volume 47:Number 4(2009)
• Page Start: 871
• Page End: 875
• Publication Date: 2009-10
• Subjects: cell motility -- luminescence -- 96-well plate; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000113250
• Languages: English
• ISSNs: 0736-6205

Abstract

Directional motility assays make use of Boyden chambers or Transwell culture inserts with porous membranes that separate cells seeded in the upper chamber from a chemoattractant supplied in a lower chamber. These assays are often time-consuming and are associated with several limitations due to manual counting and inconsistent results; low signal-to-noise ratio and fluorescence interference; and high cost and the need for specific equipment. Here, we describe a simple, direct, and easy ATP luminescence–based motility assay (ALMA), which can be used for 96-well plate quantification.