Transcriptomic analysis of mechanism of melanoma cell death induced by photothermal therapy. Issue 8 (26th May 2021)

• Record Type: Journal Article
• Authors: Yan, Guorong; Shi, Lei; Zhang, Fuhe; Luo, Min; Zhang, Guolong; Liu, Pei; Liu, Kaili; Chen, Wei R.; Wang, Xiuli
• Is Part Of: Journal of biophotonics. Volume 14:Issue 8(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-05-26
• Subjects: cell death -- immune microenvironment -- melanoma -- photothermal therapy -- transcriptome; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.202100034
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Melanoma is a malignancy with poor prognosis. Its incidence rate has been on the rise and it poses high health and economic challenges to different populations. Photothermal therapy (PTT) served as an effective local therapy in treating various tumors, particularly cutaneous carcinoma like melanoma. To fully understand the mechanisms of tumor cell death induced by PTT, we investigated gene expression and immune cells compositions of B16‐F10 tumors after PTT treatment. A total of 256 differentially expressed genes (DEGs) were identified, with 215 being downregulated and 41 upregulated by PTT. Functional annotation showed that most DEGs involved in immune response and inflammatory response. Immune cells compositions inference revealed changes in many immune cells including regulatory T cells, M2 macrophage and B cells after PTT treatment. Our results help delineate the mechanism of cell death at the transcriptional level triggered by non‐invasive PTT treatment of melanoma without exogenous light absorbing agents. Abstract : Photothermal therapy (PTT) exhibited a good outcome on melanoma. Genes expressions and Immune cell compositions were quantified after PTT treatment. Most of differentially expressed genes involved in immune response and inflammatory response. M2 macrophage composition was decreased after PTT treatment.