CD34+ STEM CELL MOBILISATION IN MS TREATMENT AND RELAPSE. Issue 10 (9th September 2014)

• Record Type: Journal Article
• Authors: Witherick, Jonathan; Virgo, Paul; Redondo, Juliana; Cottrell, David; Scolding, Neil; Rice, Claire
• Is Part Of: Journal of neurology, neurosurgery and psychiatry. Volume 85:Issue 10(2014)
• Page Start: e4
• Page End: e4
• Publication Date: 2014-09-09
• Subjects: EPILEPSY; Neurology -- Periodicals; Nervous system -- Surgery -- Periodicals; Psychiatry -- Periodicals; 616.8
• DOI: 10.1136/jnnp-2014-309236.153
• Languages: English
• ISSNs: 0022-3050

Abstract

Abstract : Release of CD34+ haematopoietic stem cells (HSC) into the peripheral circulation has been demonstrated in patients receiving anti-CD49d monoclonal antibody for the treatment of multiple sclerosis (MS). It is not known whether these cells are mobilised during an acute MS relapse or by treatment with other disease-modifying drugs (DMDs). We analysed peripheral blood samples from patients with MS including those on disease modifying therapy and during acute relapses. Circulating numbers of CD34/133+ cells were determined using flow cytometry. A novel, multi-channel flow cytometric approach was developed to identify multipotent mesenchymal stromal cells (MSCs). The finding of mobilisation of CD34+ HSCs with anti-CD49d antibody infusion was replicated. Increases in circulating numbers of CD34+ HSCs were also observed in patients treated with glatiramer acetate and in MS patients experiencing an acute relapse, irrespective of ongoing treatment with DMDs. CD133+ subsets were also increased in all groups. Circulating MSCs were not detected in the peripheral blood of patients or controls. Our results suggest a role for HSC mobilisation during treatment for MS and in acute relapse. Further work will examine the role of these cells in the pathogenesis of MS and endogenous repair.