Mitochondria protection with ginkgolide B-loaded polymeric nanocapsules prevents diethylnitrosamine-induced hepatocarcinoma in rats. (March 2014)

• Record Type: Journal Article
• Authors: Ghosh, Swarupa; Dungdung, Sandhya Rekha; Choudhury, Somsubhra Thakur; Chakraborty, Somsuta; Das, Nirmalendu
• Is Part Of: Nanomedicine. Volume 9:Number 3(2014)
• Page Start: 441
• Page End: 456
• Publication Date: 2014-03
• Subjects: COX-2 -- diethylnitrosamine -- ginkgolide B -- hepatocellular carcinoma -- inducible NOS -- mitochondria -- nanocapsule -- NF-κB -- ROS -- VEGF; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.13.56
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Hepatocellular carcinoma (HCC) has no successful pharmacotherapeutic remedy. The aim of this study was to ascertain whether ginkgolide B (GB)-loaded polymeric nanocapsules can prevent diethylnitrosamine (DEN)-induced HCC in rats.Materials & methods: GB was fabricated in two types of nanocapsules of which one was polyethylene glycol coated (N1GB) and the other was uncoated (N2GB). These nanocapsules were orally gavaged during DEN-induced HCC development in rats.Results: Nanocapsulation of GB enabled aqueous suspension and slow time-dependent release of the compound. Anticarcinogenic potential of N2GB was reflected by its ability in the management of DEN-induced reactive oxygen species generation, mitochondrial dysfunction, p53, NF-κB, inducible nitric oxide synthase, COX-2 and VEGF expressions, and induction of apoptosis in cancer cells in the rat liver.Conclusion: Positive zeta-potential on N2GB surface might have offered higher hepatic accumulation of GB, especially at the electron-dense organelle mitochondria. Mitochondria protection against DEN-induced oxidative damage ensured HCC prevention. Original submitted 27 June 2012; Revised submitted 4 December 2012; Published online 7 June 2013