Applicability of self-obtained urine and vaginal samples for HPV-16, -18, -31 and -45 cervical cancer screening in pregnancy: a pilot cross-sectional study. (April 2014)

• Record Type: Journal Article
• Authors: Mantzana, Paraskevi; Pournaras, Spyros; Skentou, Chara; Deligeoroglou, Efthimios; Katsioulis, Antonios; Antonakopoulos, George; Hadjichristodoulou, Christos; Tsakris, Athanassios; Messinis, Ioannis E; Daponte, Alexandros
• Is Part Of: Future virology. Volume 9:Number 4(2014)
• Page Start: 385
• Page End: 395
• Publication Date: 2014-04
• Subjects: HPV-16 -- HPV-18 -- HPV-31 -- HPV-45 -- HPV pregnancy -- HPV urine -- HPV vagina -- screening -- self-sampling; Virology -- Periodicals; Virus diseases -- Treatment -- Periodicals; 616.9101
• DOI: 10.2217/fvl.14.11
• Languages: English
• ISSNs: 1746-0794

Abstract

ABSTRACT: Aim: To conduct a pilot cross-sectional study to evaluate the rates of detection of four common high-risk HPV (hr-HPV) types using first-void urine paired with vaginal self-obtained samples in a nonvaccinated population of pregnant women. We also aimed to compare these results with a matched nonpregnant group in order to test the applicability of self-sampled hr-HPV cervical cancer screening during antenatal visits.Materials & methods: Samples from 550 pregnant women were subjected to hr-HPV-16, -18, -31 and -45 type detection by inhouse PCR and compared with 250 paired urine, vaginal and cervical samples from an age-matched cohort of nonpregnant women.Results: Comparing overall hr-HPV prevalence in urine and vaginal samples between pregnant (15 out of 550; 2.7%) and nonpregnant women (eight out of 250; 3.2%) for each HPV type revealed no significant differences. All paired urine/vaginal samples were both positive for the same type of hr-HPV and there was no positive urine sample with the other samples being negative.Conclusion: hr-HPV detection in pregnant women using self-obtained urine and vaginal samples seems to be a feasible cervical cancer screening method.