Oral human papillomavirus infection in England and associated risk factors: a case–control study. Issue 8 (19th August 2018)

• Record Type: Journal Article
• Authors: Hearnden, Vanessa; Murdoch, Craig; D'Apice, Katy; Duthie, Susan; Hayward, Nicholas J; Powers, Hilary Jane
• Is Part Of: BMJ open. Volume 8:Issue 8(2018)
• Publication Date: 2018-08-19
• Subjects: HPV -- head and neck cancer -- oncology -- folate; Medicine -- Research -- Periodicals; 610.72
• DOI: 10.1136/bmjopen-2018-022497
• Languages: English
• ISSNs: 2044-6055

Abstract

Abstract : Objectives: This study was conducted to determine the prevalence of and associated risk factors for infection with oral high-risk human papillomavirus (HR-HPV) in adult participants within England, and to explore any association with oral mucosal buccal epithelial cell and whole blood folate concentration. Design: This was an observational study to determine oral HR-HPV prevalence in the study population. A case–control study was performed to explore the association between infection and folate status. Setting: This study was conducted in Sheffield, UK, between April 2013 and August 2014. Participants: Seven hundred participants, aged 18–60 years, were recruited from university students (n=179), university and hospital staff (n=163), dental hospital patients (n=13), Sexual Health Sheffield patients (n=122) and the general public (n=223). Interventions: Participants completed a lifestyle and sexual behaviour questionnaire, provided an oral rinse and gargle sample for the detection of oral HR-HPV and an oral mucosal buccal epithelial cell sample for the measurement of oral mucosal buccal epithelial cell folate. A blood sample was collected for measurement of whole blood folate concentration. Outcome measures: The prevalence of oral HR-HPV infection in the study population was the primary outcome measure. Secondary outcome measures included associations between risk factors, folate status and infection. Results: The prevalence of oral HR-HPV infection in this cohort was 2.2% (15/680) with 0.7% (5/680) positive for HPV16 or HPV18. Twenty samples were excluded due to insufficient material for HPV detection. Participants with oral HR-HPV infection were more likely to be a former smoker, and have a greater number of sexual and oral sexual partners. Folate status was not linked to likelihood of HPV infection. Conclusions: The prevalence of oral infection with HR-HPV in adult men and women in Sheffield in the North of England was low. Smoking and sexual behaviour were associated with HR-HPV positivity. Trial registration number: ID14106.