Evaluation of salivary anti-Salmonella typhi lipopolysaccharide IgA ELISA for serodiagnosis of typhoid fever in children. Issue 3 (3rd January 2012)

• Record Type: Journal Article
• Authors: Zaka-ur-Rab, Zeeba; Abqari, Shaad; Shahab, Tabassum; Islam, Najmul; Shukla, Indu
• Is Part Of: Archives of disease in childhood. Volume 97:Issue 3(2012)
• Page Start: 236
• Page End: 238
• Publication Date: 2012-01-03
• Subjects: Children -- Diseases -- Periodicals; Infants -- Diseases -- Periodicals; 618.920005
• DOI: 10.1136/adc.2011.300622
• Languages: English
• ISSNs: 0003-9888

Abstract

Abstract : This observational study was conducted to determine the diagnostic accuracy of ELISA for the detection of anti- Salmonella typhi lipopolysaccharide (LPS) salivary immunoglobulin A (IgA) antibodies in 37 children with culture confirmed typhoid, 30 febrile controls with an alternative diagnosis and 30 healthy controls. The test was positive in 33/37 (89.2%) cases of typhoid, but negative in all patients in the two control groups. Maximum absorbance of anti-LPS IgA was observed during the second and third weeks of typhoid, with a progressive decline thereafter. The sensitivity of ELISA was 71.4%, 100%, 100%, 9.1% and 0%, in first, second, third, fourth and fifth week of illness, respectively. Further large scale studies measuring salivary anti-LPS IgA antibodies are needed to confirm the potential of saliva-based serology in children with suspected typhoid.