Interleukin 4 increases human synovial cell expression of VCAM-1 and T cell binding. Issue 9 (September 1994)

• Record Type: Journal Article
• Authors: Shimada, H; Eguchi, K; Ueki, Y; Nakashima, M; Yamashita, I; Kawabe, Y; Sakai, M; Ida, H; Aoyagi, T; Nagataki, S
• Is Part Of: Annals of the rheumatic diseases. Volume 53:Issue 9(1994)
• Page Start: 601
• Page End: 607
• Publication Date: 1994-09
• Subjects: Rheumatism -- Periodicals; 616.723005
• DOI: 10.1136/ard.53.9.601
• Languages: English
• ISSNs: 0003-4967

Abstract

Abstract : OBJECTIVE--The effects were studied of interleukin 4 (IL-4) on T cell-synovial cell adhesion and on the expression of adhesion molecules on the surface of synovial fibroblast-like cells. METHODS--The adhesion of T cells toward the synovial cells were measured by 51chromium-labelled adhesion assay. The expression of adhesion molecules on synovial cells were analysed by flowcytometry. RESULTS--Stimulation of synovial cells with IL-4 increased T cell-synovial cells adhesion in a time- and dose-dependent manner. IL-4 considerably enhanced the expression of VCAM-1 on the surface of synovial cells, but not the expression of ICAM-1 and ELAM-1. The combination of IL-1 beta and IL-4 had no effect on the expression of ICAM-1 or VCAM-1 on the surface of synovial cells. The increased adhesion of T cells to IL-4 stimulated synovial cells was inhibited significantly by adding anti-VCAM-1 or anti-CD29 monoclonal antibody. Furthermore, anti-VLA-4 alpha or the combination of anti-VLA-4 alpha and anti-VCAM-1 antibodies blocked completely T-cell binding to IL-4 stimulated synovial cells. CONCLUSIONS--These results suggest that the increased adhesion of T cells to IL-4-stimulated synovial cells is mediated by VLA-4/VCAM-1 pathway.