Visualization of Intra‐neuronal Motor Protein Transport through Upconversion Microscopy. (28th May 2019)

• Record Type: Journal Article
• Authors: Zeng, Xiao; Chen, Shuo; Weitemier, Adam; Han, Sanyang; Blasiak, Agata; Prasad, Ankshita; Zheng, Kezhi; Yi, Zhigao; Luo, Baiwen; Yang, In‐Hong; Thakor, Nitish; Chai, Chou; Lim, Kah‐Leong; McHugh, Thomas J.; All, Angelo H.; Liu, Xiaogang
• Is Part Of: Angewandte Chemie. Volume 131:Number 27(2019)
• Page Start: 9363
• Page End: 9369
• Publication Date: 2019-05-28
• Subjects: Axon-Transport -- Dynein -- Einzelpartikel-Auflösung -- Upconversion-Mikroskopie -- Weitfeld-Beleuchtung; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201904208
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real‐time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single‐particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra‐axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery. Abstract : Motorüberwachung : Die Echtzeit‐Überwachung der Bewegung von Motorproteinen durch konventionelle Fluoreszenzmikroskopie hat aufgrund der geringen Eindringtiefe des Lichts und der komplexen Instrumentierung signifikante Einschränkungen. Um diese Schwierigkeiten zu umgehen, wurde Upconversion‐Mikroskopie für die Echtzeit‐Visualisierung der Verfolgung von Dynein in Axonen und mehrschichtigen Zellkulturen eingesetzt.