A Small‐Molecule AIE Chromosome Periphery Probe for Cytogenetic Studies. Issue 26 (6th April 2020)

• Record Type: Journal Article
• Authors: Wu, Ming‐Yu; Leung, Jong‐Kai; Liu, Li; Kam, Chuen; Chan, Kelvin Yuen Kwong; Li, Ronald A.; Feng, Shun; Chen, Sijie
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 26(2020)
• Page Start: 10327
• Page End: 10331
• Publication Date: 2020-04-06
• Subjects: aggregation-induced emission -- centromeres -- chromosome analysis -- chromosome periphery -- fluorescence imaging; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201916718
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The chromosome periphery (CP) is a complex network that covers the outer surface of chromosomes. It acts as a carrier of nucleolar components, helps maintain chromosome structure, and plays an important role in mitosis. Current methods for fluorescence imaging of CP largely rely on immunostaining. We herein report a small‐molecule fluorescent probe, ID‐IQ, which possesses aggregation‐induced emission (AIE) property, for CP imaging. By labelling the CP, ID‐IQ sharply highlighted the chromosome boundaries, which enabled rapid segmentation of touching and overlapping chromosomes, direct identification of the centromere, and clear visualization of chromosome morphology. ID‐IQ staining was also compatible with fluorescence in situ hybridization and could assist the precise location of the gene in designated chromosome. Altogether, this study provides a versatile cytogenetic tool for improved chromosome analysis, which greatly benefits the clinical diagnostic testing and genomic research. Abstract : Peripheral vision : A small‐molecule fluorescent probe with aggregation‐induced emission was developed for chromosome periphery imaging, which facilitates the segmentation of touching or overlapping chromosome clusters, identification of the centromere, and visualization of chromosome morphology. Its compatibility with FISH assays makes it a practical cytogenetic tool in assisting the precise location of a gene in designated chromosomes.