Characterisation of hexon and fibre genes of a novel strain of adenovirus involved in epidemic keratoconjunctivitis. Issue 1 (23rd December 2003)

• Record Type: Journal Article
• Authors: Adhikary, A K; Inada, T; Numaga, J; Suzuki, E; Ushijima, H; Banik, U; Mukouyama, A; Matsuno, S; Okabe, N
• Is Part Of: Journal of clinical pathology. Volume 57:Issue 1(2004)
• Page Start: 95
• Page End: 97
• Publication Date: 2003-12-23
• Subjects: adenovirus -- restriction endonuclease analysis -- sequence analysis; AA, amino acid -- Ad, adenovirus -- EKC, epidemic keratoconjunctivitis -- HVR, hypervariable region; Pathology -- Periodicals; Pathology, Molecular -- Periodicals; 616.0705
• DOI: 10.1136/jcp.57.1.95
• Languages: English
• ISSNs: 0021-9746

Abstract

Abstract : Aims: To characterise a novel strain (M86) of adenovirus (Ad) involved in epidemic keratoconjunctivitis (EKC). Methods/Results: The virus strain was neutralised by antisera to both Ad35 and Ad11. Restriction endonuclease analysis of genomic DNA showed 98% and 88% homology with Ad11 and Ad35, respectively. The deduced amino acid sequence of the hypervariable regions of (HVRs) of the hexon gene showed a higher homology with Ad35 (94.4%) than with Ad11 (83.7%). However, it was 100% homologous to Ad35 in HVRs 1, 2, 3, and 6 and to Ad11 in HVRs 4 and 6. In the fibre knob, the isolate was more homologous to Ad11 (99.4%) than to Ad35 (29.1%). Conclusion: This novel strain of adenovirus showed similarities with both Ad11 and Ad35. The isolation of a novel strain like Ad35+11 is important because of its association with EKC.