Detection and quantification of house mouse Eimeria at the species level – Challenges and solutions for the assessment of coccidia in wildlife. (December 2019)

• Record Type: Journal Article
• Authors: Jarquín-Díaz, Víctor Hugo; Balard, Alice; Jost, Jenny; Kraft, Julia; Dikmen, Mert Naci; Kvičerová, Jana; Heitlinger, Emanuel
• Is Part Of: International journal for parasitology. Volume 10(2019)
• Page Start: 29
• Page End: 40
• Publication Date: 2019-12
• Subjects: Eimeria -- Coccidia -- House mice -- Diagnostic PCR -- Species-specific prevalence -- qPCR; Parasites -- Periodicals; Parasitology -- Periodicals; Animals -- Periodicals; Wildlife diseases -- Periodicals; Parasites -- Periodicals; Animals, Wild -- Periodicals; Animals; Parasites; Parasitology; Wildlife diseases; Periodicals; 591.7857
• DOI: 10.1016/j.ijppaw.2019.07.004
• Languages: English
• ISSNs: 2213-2244

Abstract

Abstract: Detection and quantification of coccidia in studies of wildlife can be challenging. Therefore, prevalence of coccidia is often not assessed at the parasite species level in non-livestock animals. Parasite species – specific prevalences are especially important when studying evolutionary questions in wild populations. We tested whether increased host population density increases prevalence of individual Eimeria species at the farm level, as predicted by epidemiological theory. We studied free-living commensal populations of the house mouse ( Mus musculus ) in Germany, and established a strategy to detect and quantify Eimeria infections. We show that a novel diagnostic primer targeting the apicoplast genome (Ap5) and coprological assessment after flotation provide complementary detection results increasing sensitivity. Genotyping PCRs confirm detection in a subset of samples and cross-validation of different PCR markers does not indicate bias towards a particular parasite species in genotyping. We were able to detect double infections and to determine the preferred niche of each parasite species along the distal-proximal axis of the intestine. Parasite genotyping from tissue samples provides additional indication for the absence of species bias in genotyping amplifications. Three Eimeria species were found infecting house mice at different prevalences: Eimeria ferrisi (16.7%; 95% CI 13.2–20.7), E. falciformis (4.2%; 95% CI 2.6–6.8) and E. vermiformis (1.9%; 95% CI 0.9–3.8). We also find that mice in dense populations are more likely to be infected with E. falciformis and E. ferrisi . We provide methods for the assessment of prevalences of coccidia at the species level in rodent systems. We show and discuss how such data can help to test hypotheses in ecology, evolution and epidemiology on a species level. Graphical abstract: Image 1 Highlights: Flotation and PCR provide complementary results for Eimeria detection in house mice. Genotyping PCRs confirm detections. E. ferrisi, E. falciformis, and E. vermiformis infect natural populations of M. musculus. Double infections and preferentially infected tissues could be identified using qPCR. Potential virulence prevalence trade-off for Eimeria of house mice.