Molecular characterization of Cryptosporidium and Enterocytozoon bieneusi in Père David's deer (Elaphurus davidianus) from Shishou, China. (December 2019)

• Record Type: Journal Article
• Authors: Xie, Fujie; Zhang, Zhenjie; Zhao, Aiyun; Jing, Bo; Qi, Meng; Wang, Rongjun
• Is Part Of: International journal for parasitology. Volume 10(2019)
• Page Start: 184
• Page End: 187
• Publication Date: 2019-12
• Subjects: Cryptosporidium -- Enterocytozoon bieneusi -- Genotype -- SSU rRNA -- Père David's deer; Parasites -- Periodicals; Parasitology -- Periodicals; Animals -- Periodicals; Wildlife diseases -- Periodicals; Parasites -- Periodicals; Animals, Wild -- Periodicals; Animals; Parasites; Parasitology; Wildlife diseases; Periodicals; 591.7857
• DOI: 10.1016/j.ijppaw.2019.09.001
• Languages: English
• ISSNs: 2213-2244

Abstract

Abstract: Cryptosporidium and Enterocytozoon bieneusi are important intestinal pathogens that infect humans and various animals. Few reports are available regarding the infections of the two pathogens in Père David's deer. In this study, polymerase chain reaction (PCR) confirmed Cryptosporidium infection in two (1.6%) and E. bieneusi in 45 (35.2%) of 128 fecal samples collected from Père David's deer in the National Nature Reserve of Shishou, Hubei Province, China. C. parvum (n = 1) and Cryptosporidium deer genotype (n = 1) were identified using the small subunit rRNA (SSU rRNA) gene. The C. parvum was further subtyped as IIdA20G1 by sequencing analysis of the 60-kDa glycoprotein (gp60) gene. The identity of E. bieneusi was confirmed by an internal transcribed spacer (ITS) gene; the HLJD-V (n = 42) and MWC_d1 (n = 3) genotypes were identified, with the former clustering in group 2 and the latter in group 1. These data suggest that the Père David's deer were infected with host-specific and/or zoonotic genotypes of these pathogens, implicating Père David's deer could be a potential source of human Cryptosporidium infection. Graphical abstract: Image 1 Highlights: C. parvum subtype IIdA20G1 was firstly identified in Pere David's deer. Genotypes HLJD-V and MWC_d1 were detected from 45 E. bieneusi -positive samples. The present study implicated Père David's deer could be a potential source of human infection.