Similar in vitro effects and pulp regeneration in ectopic tooth transplantation by basic fibroblast growth factor and granulocyte‐colony stimulating factor. (25th March 2014)

• Record Type: Journal Article
• Authors: Takeuchi, N; Hayashi, Y; Murakami, M; Alvarez, FJ; Horibe, H; Iohara, K; Nakata, K; Nakamura, H; Nakashima, M
• Is Part Of: Oral diseases. Volume 21:Number 1(2015:Jan.)
• Page Start: 113
• Page End: 122
• Publication Date: 2014-03-25
• Subjects: basic fibroblast growth factor -- granulocyte‐colony stimulating factor -- pulp regeneration -- homing/migration factor; Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.12227
• Languages: English
• ISSNs: 1354-523X

Abstract

Abstract : Objectives: Granulocyte‐colony stimulating factor (G‐CSF) has been shown to have combinatorial trophic effects with dental pulp stem cells for pulp regeneration. The aim of this investigation is to examine the effects of basic fibroblast growth factor (bFGF) in vitro and in vivo compared with those of G‐CSF and to assess the potential utility of bFGF as an alternative to G‐CSF for pulp regeneration. Materials and methods: Five different types of cells were examined in the in vitro effects of bFGF on cell migration, proliferation, anti‐apoptosis, neurite outgrowth, angiogenesis, and odontogenesis compared with those of G‐CSF. The in vivo regenerative potential of pulp tissue including vasculogenesis and odontoblastic differentiation was also compared using an ectopic tooth transplantation model. Results: Basic fibroblast growth factor was similar to G‐CSF in high migration, proliferation and anti‐apoptotic effects and angiogenic and neurite outgrowth stimulatory activities in vitro . There was no significant difference between bFGF and G‐CSF in the regenerative potential in vivo . Conclusions: The potential utility of bFGF for pulp regeneration is demonstrated as a homing/migration factor similar to the influence of G‐CSF.