Exogenous Dietary Ketone Ester Decreases Body Weight and Adiposity in Mice Housed at Thermoneutrality. Issue 8 (2nd July 2020)

• Record Type: Journal Article
• Authors: Deemer, Sarah E.; Davis, Rachel A. H.; Roberts, Brandon M.; Smith, Daniel L.; Koutnik, Andrew P.; Poff, Angela M.; D'Agostino, Dominic P.; Plaisance, Eric P.
• Is Part Of: Obesity. Volume 28:Issue 8(2020)
• Page Start: 1447
• Page End: 1455
• Publication Date: 2020-07-02
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.22855
• Languages: English
• ISSNs: 1930-7381

Abstract

Abstract : Objective: The aim of this study was to examine the effects of a ketone ester (KE)‐supplemented diet on energy expenditure (EE) and adiposity in mice housed at 23 °C versus thermoneutrality (30 °C), in which sympathetic nervous system activity is diminished. Methods: Thirty‐two 10‐week‐old male C57BL/6J mice were assigned to 1 of 4 groups ( n = 8 per group): 30% KE diet + 23 °C (KE23), control (CON) diet + 23 °C (CON23), 30% KE diet + 30 °C (KE30), or CON diet + 30 °C (CON30). CON mice were pair‐fed to the average intake of mice consuming the KE diet (ad libitum) for 8 weeks. Body composition and components of energy balance were measured at completion of the study. Results: CON23 (mean ± SD, 26.0 ± 1.6 g) and CON30 (29.7 ± 1.4 g) mice weighed more than KE groups ( P < 0.03 for both) and were also different from each other (CON23 vs. CON30, P < 0.01). However, KE23 (23.4 ± 2.7 g) and KE30 (23.1 ± 1.9 g) mice were not different in body weight. As expected, food intake at 30 °C (2.0 ± 0.3 g/d) was lower than at 23 °C (2.6 ± 0.3 g/d, P < 0.01). Diet did not influence resting and total EE, but mice housed at 30 °C had lower EE compared with mice at 23 °C ( P < 0.01). Conclusions: Dietary KEs attenuate body weight gain at standard (23 °C) and thermoneutral (30 °C) housing temperatures, and this effect is not mediated by increased EE under these conditions.