A WRKY transcription factor confers aluminum tolerance via regulation of cell wall modifying genes. Issue 8 (13th March 2020)

• Record Type: Journal Article
• Authors: Li, Chun Xiao; Yan, Jing Ying; Ren, Jiang Yuan; Sun, Li; Xu, Chen; Li, Gui Xin; Ding, Zhong Jie; Zheng, Shao Jian
• Is Part Of: Journal of integrative plant biology. Volume 62:Issue 8(2020)
• Page Start: 1176
• Page End: 1192
• Publication Date: 2020-03-13
• Subjects: Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12888
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: Modification of cell wall properties has been considered as one of the determinants that confer aluminum (Al) tolerance in plants, while how cell wall modifying processes are regulated remains elusive. Here, we present a WRKY transcription factor WRKY47 involved in Al tolerance and root growth. Lack of WRKY47 significantly reduces, while overexpression of it increases Al tolerance. We show that lack of WRKY47 substantially affects subcellular Al distribution in the root, with Al content decreased in apoplast and increased in symplast, which is attributed to the reduced cell wall Al‐binding capacity conferred by the decreased content of hemicellulose I in the wrky47‐1 mutant. Based on microarray, real time‐quantitative polymerase chain reaction and chromatin immunoprecipitation assays, we further show that WRKY47 directly regulates the expression of EXTENSIN‐LIKE PROTEIN ( ELP ) and XYLOGLUCAN ENDOTRANSGLUCOSYLASE‐HYDROLASES17 ( XTH17 ) responsible for cell wall modification. Increasing the expression of ELP and XTH17 rescued Al tolerance as well as root growth in wrky47‐1 mutant. In summary, our results demonstrate that WRKY47 is required for root growth under both normal and Al stress conditions via direct regulation of cell wall modification genes, and that the balance of Al distribution between root apoplast and symplast conferred by WRKY47 is important for Al tolerance. Abstract : We show that WRKY47 promotes root cell elongation possibly by directly regulating the expression of ELP and XTH17 during normal root development, while under Al stress, such regulation could maintain the balance of Al distribution between root apoplast and symplast, thereby enhancing Al tolerance.