C10 Behavioural phenotyping of minipigs transgenic for the huntington gene. (13th September 2016)

• Record Type: Journal Article
• Authors: Schuldenzucker, Verena; Schubert, Robin; Freisfeld, Frauke; Rieke, Lorena; Matheis, Tamara; Motlik, Jan; Schramke, Sarah; Reilmann, Ralf
• Is Part Of: Journal of neurology, neurosurgery and psychiatry. Volume 87(2016)Supplement 1
• Page Start: A30
• Page End: A30
• Publication Date: 2016-09-13
• Subjects: animal models -- minipig -- HD -- phenotyping -- behavioural -- preclinical research; Neurology -- Periodicals; Nervous system -- Surgery -- Periodicals; Psychiatry -- Periodicals; 616.8
• DOI: 10.1136/jnnp-2016-314597.90
• Languages: English
• ISSNs: 0022-3050

Abstract

Abstract : Background: While several novel therapeutic approaches for HD are in development, resources to conduct clinical trials are limited. Large animal models have been proposed to improve assessment of safety, tolerability and especially to increase translational reliability of efficacy signals obtained in preclinical studies. They may thus help to select candidates for translation to human studies. We here introduce a battery of novel tests designed to assess the motor, cognitive and behavioural phenotype of a transgenic (tg) HD minipig model. Methods: A group of tgHD and wildtype (wt) Libechov minipigs (n = 36) was available for assessment with (1) a gait test using the GAITRite® automated acquisition system, (2) a hurdle-test, (3) a tongue coordination test, (4) a startbox back and forth test and (5) a dominance test. Performance of all tests and definition of measures obtained is presented. Results: Minipigs were able to learn performance of all tests. All tests were safe, well tolerated and feasible. Exploratory between group comparisons showed no differences between groups of tgHD and wt minipigs assessed, but low variability within and between groups. Conclusions: The data shows that the tests presented are safe, well tolerated and all measures defined can be assessed. Prospective longitudinal application of these tests is warranted to determine their test-retest reliability, sensitivity and validity in assessing motor, cognitive and behavioural features of tg and wt minipigs. Acknowledgement: Funded by the CHDI Foundation.