Tanshinone IIA protects mice against atherosclerotic injury by activating the TGF-β/PI3K/Akt/eNOS pathway. Issue 4 (June 2020)

• Record Type: Journal Article
• Authors: Wang, Junyan; He, Xinyong; Chen, Wenna; Zhang, Ni; Guo, Junfu; Liu, Jingjing; Zhang, Lin; Sun, Hongwei; Jia, Lianqun; Yang, Guanlin
• Is Part Of: Coronary artery disease. Volume 31:Issue 4(2020:Jun.)
• Publication Date: 2020-06
• Subjects: atherosclerosis -- endothelial nitric oxide synthase -- phosphatidylinositol 3-kinase/protein kinase B -- tanshinone IIA -- transforming growth factor β; Coronary heart disease -- Periodicals; Coronary Disease -- Indexes; Coronary Disease -- Periodicals; 616.123005
• DOI: 10.1097/MCA.0000000000000835
• Languages: English
• ISSNs: 0954-6928

Abstract

Abstract : Objective: Explored the mechanism of action of tanshinone IIA (TIIA) against atherosclerosis. Methods: ApoE −/− mice were divided into two groups of 10: model and TIIA. A control group of 10 wild-type mice was created. ApoE −/− mice were fed a high-fat diet for 12 weeks. The TIIA group received TIIA once daily. Mice were anesthetized, blood collected by cardiac puncture, and the aortic sinus/arch collected for histology and molecular studies, respectively. Results: Mice intima in the model group had large areas of plaque formation, serum levels of total cholesterol (TC), triglycerides, and low-density lipoprotein-cholesterol (LDL-C) increased significantly, and high-density lipoprotein-cholesterol (HDL-C) levels decreased significantly in the model group after 12 weeks. Staining [hematoxylin and eosin (H&E), Oil-Red-O] showed that the aorta had lesions, a higher degree of plaque formation, and considerable lipid deposition in model-group mice. After TIIA treatment, expression of HDL-C was increased significantly and that of TC, triglycerides and LDL-C decreased significantly, and plaque size and lipid deposition improved obviously. Analyses of protein phosphorylation in aortic tissue suggested that the transforming growth factor (TGF)-β/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/endothelial nitric oxide synthase (eNOS) pathway was activated in TIIA-treated mice. Conclusion: TIIA can lower levels of serum lipids, stabilize atherosclerotic plaques, reduce endothelial injury, and inflammatory damage by activation of the TGF-β/PI3K/Akt/eNOS pathway.