Deuterium solid‐state NMR quadrupolar order rotating frame relaxation with applications to amyloid‐β fibrils. (10th November 2020)

• Record Type: Journal Article
• Authors: Vugmeyster, Liliya; Ostrovsky, Dmitry
• Other Names: Laurencin Danielle guestEditor.; Ashbrook Sharon guestEditor.
• Is Part Of: Magnetic resonance in chemistry. Volume 59:Number 9/10(2021)
• Page Start: 853
• Page End: 863
• Publication Date: 2020-11-10
• Subjects: 2H solid‐state NMR -- NMR -- protein dynamics -- quadrupolar order -- rotating frame; Nuclear magnetic resonance spectroscopy -- Periodicals; Chemistry, Organic -- Periodicals; Magnetic resonance -- Periodicals; 538.36
• DOI: 10.1002/mrc.5114
• Languages: English
• ISSNs: 0749-1581

Abstract

Abstract: We describe a new method for measuring molecular dynamics based on the deuterium solid‐state nuclear magnetic resonance (NMR) quadrupolar order rotating frame relaxation rate R 1 ρ, Q under static conditions. The observed quadrupolar order coherence is created using the broad‐band Jeener–Broekaert excitation and is locked with a weak radio frequency (RF) field. We describe the experimental and theoretical approaches and show applications to a selectively deuterated valine side chain of the phosphorylated amyloid‐β (1–40) fibrils phosphorylated at the serine‐8 position. The R 1 ρ, Q rate is sensitive to the rotameric exchange mode. For biological samples, the low spin‐lock field in the 5‐ to 10‐kHz range has the advantage of avoiding sample heating and dehydration. Thus, it provides an alternative to approaches based on single‐quantum coherence, which require larger spin‐lock fields. Abstract : We describe a new method for measuring molecular dynamics based on the deuterium solid‐state NMR quadrupolar order rotating frame relaxation rate R 1 ρ, Q under static conditions. We present the experimental and theoretical approaches and show applications to a selectively deuterated valine side chain of the phosphorylated amyloid‐β fibrils.