Identification and monitoring of Copy Number Variants (CNV) in monoclonal gammopathy. Issue 5 (3rd June 2021)

• Record Type: Journal Article
• Authors: Sallustio, Fabio; Curci, Claudia; Solimando, Antonio Giovanni; Leone, Patrizia; Pontrelli, Paola; Gesualdo, Loreto; Vacca, Angelo; Racanelli, Vito; Gallone, Anna
• Is Part Of: Cancer biology & therapy. Volume 22:Issue 5/6(2021)
• Page Start: 404
• Page End: 412
• Publication Date: 2021-06-03
• Subjects: MGUS -- MM -- CNVs detection -- digital PCR; 616.99406
• DOI: 10.1080/15384047.2021.1946458
• Languages: English
• ISSNs: 1538-4047

Abstract

ABSTRACT: Monoclonal gammopathy of undetermined significance (MGUS) represents the pre-clinical stage of Multiple Myeloma (MM) with the 5% of MGUS progresses to MM. Although the progression from MGUS to MM has not been completely characterized, it is possible to monitor the DNA modifications of patients diagnosed with MGUS to detect early specific genomic abnormalities, including copy number variations (CNV). The CNVs of chromosome 1q and chromosome 13q are associated with a worse prognosis in MM. In the present study, we showed that it is possible to monitor the 1q21 gain and 13q deletion frequencies in gDNA using digital PCR. The CNV analysis of three cell lines with a well-characterized cytogenetic profile were compared with measures performed by a real-time PCR approach and with a digital PCR approach. Then, we analyzed CNVs in CD138 + plasma cells isolated from bone marrow of MGUS and MM patients. Our results show that digital PCR and targeted DNA monitoring represent a specific and accurate technique for the early detection of specific genomic abnormalities both in MM and in MGUS patients. Our results could represent a remarkable advancement in MM and MGUS diagnosis and in CNV analysis for the evaluation of the risk of progression from MGUS to MM.