Antioxidant Potential of Parsley Leaf (Petroselinum crispum) Essential Oil on Hypothyroidism and Testicular Injury in Mice Intoxicated by Carbon Tetrachloride. (30th August 2021)

• Record Type: Journal Article
• Authors: Badr, Gehan M.; Algefare, Abdulmohsen I.; Alfwuaires, Manal A.
• Other Names: Ahamed Maqusood Academic Editor.
• Is Part Of: BioMed research international. Volume 2021(2021)
• Publication Date: 2021-08-30
• Subjects: Medicine -- Periodicals; Biology -- Periodicals; Biotechnology -- Periodicals; Life sciences -- Periodicals; 610.5
• DOI: 10.1155/2021/9989174
• Languages: English
• ISSNs: 2314-6133

Abstract

Abstract : The aim of the present study was to investigate the ameliorative potential of parsley ( Petroselinum crispum ) leaf essential oil (PO) against the detrimental effects of carbon tetrachloride (CCl4 ) on the thyroid gland and testes of mice. Twenty-four adult male mice were divided into four groups and treated for 4 weeks. The 1 st control group received 3 mL/kg olive oil intraperitoneally, twice a week followed by 0.5 mL/kg saline intragastrically daily. The 2 nd CCl4 group received CCl4 (3 mL/kg intraperitoneally, twice a week). The 3 rd PO group received PO (0.5 mL/kg intragastrically daily), while the 4 th CCl4 +PO group received CCl4 coadministrated with PO at the aforementioned doses. CCl4 group recorded significant (p < 0.05 ) reduction in the activities of antioxidant enzyme catalase (CAT) and superoxide dismutase (SOD) and significant (p < 0.05 ) increase in the lipid peroxidation end product's level malondialdehyde (MDA) in the testes and thyroid glands. Meanwhile, serum levels of testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), and thyroid hormones (thyroid-stimulating hormone (TSH), total triiodothyronine (T3), free triiodothyronine (fT3), total thyroxine (T4), and free thyroxine (fT4)) significantly decreased. Also, histopathologically, the testicular tissue showed hypospermatogenesis within irregular-shaped seminiferous tubules with prominent edema in the interstitial spaces confirming the aforementioned biochemical alterations. Treatment with PO significantly reduced the testicular and thyroid oxidative stress (p < 0.05 ) and elevated the testosterone (73.47%), FSH (92.11%), LH (33.33%), T3 (23.47%), fT3 (39.13%), T4 (27.91%), and fT4 (75%) as compared to that of CCl4 -treated group corresponding values. The PO GC/MS analysis indicated bioactive monoterpenes (major component is 1, 3, 8-mentha triene 34.48%) and phenylpropenes (major component is myristicin 21.04%). Results suggested the ameliorative effect of PO against CCl4 -induced hypogonadism in mice by suppressing oxidative stress and maintaining thyroid gland function.