Detection of phosphine resistance in major stored-product insects in Greece and evaluation of a field resistance test kit. (June 2019)

• Record Type: Journal Article
• Authors: Agrafioti, P.; Athanassiou, C.G.; Nayak, M.K.
• Is Part Of: Journal of stored products research. Volume 82(2019)
• Page Start: 40
• Page End: 47
• Publication Date: 2019-06
• Subjects: Stored product insects -- Phosphine resistance -- Resistance diagnostics -- Greece; Food -- Storage -- Periodicals; Farm produce -- Storage -- Diseases and injuries -- Periodicals; Entomology -- Periodicals; Food Contamination -- Periodicals; Food Preservation -- Periodicals; Insect Control -- Periodicals; Aliments -- Entreposage -- Périodiques; Produits agricoles -- Entreposage -- Maladies et dommages -- Périodiques; Electronic journals; 631.568
• DOI: 10.1016/j.jspr.2019.02.004
• Languages: English
• ISSNs: 0022-474X

Abstract

Abstract: The use of phosphine has been effective against a wide range of stored-product pests in different types of commodities and facilities. However, its continuous and improper use has led to resistance development in -several major insect species. Although phosphine resistance has been reported from many countries across the globe, reports from Europe have been very limited. In the present study, we determined phosphine resistance in insect populations that had been collected from a range of storages across Greece, using two different diagnostic protocols. Apart from the traditional Food and Agriculture Organization (FAO) protocol, a field test kit (known as the Detia Degesch Tolerance Test Kit, DDTTPK) was utilized, for "same day" determination of the resistance status of field collected insects. In total, 53 populations belonging to Rhyzopertha dominica, Sitophilus oryzae, Sitophilus granarius, Cryptolestes ferrugineus, Tribolium confusum, Tribolium castaneum and Oryzaephilus surinamensis were tested. For the majority of the species and populations tested, both FAO and DDPTTK provided similar results, for the susceptibility to phosphine and thus, the quick test could be used with success for an initial same day screening of phosphine resistance. Among the tested species, the populations recorded with the most frequent survival at the FAO testing dose of phosphine was that of R. dominica . The dissimilar evaluation and characterization of resistance to phosphine between diagnostic protocols is particularly important, as it poses risks in the over or underestimation of the resistance status of a given population. Our data indicate that the DDPTTK could be used to determine resistance to phosphine in the field, before the initiation of fumigations to disinfest stored commodities. Highlights: Sampling of populations of stored product beetles from Greece, indicated notable variations in the susceptibility to phosphine. From the species tested, Oryzaephilus surinamensis and Rhyzopertha dominica were more commonly found to be resistant to phosphine. A quick diagnostic test, provided similar results in the evaluation of resistance with the Food and Agriculture Organization test.