Molecular characterization of yellow catfish (Pelteobagrus fulvidraco) IRF7 suggests involvement in innate immune response. (August 2020)

• Record Type: Journal Article
• Authors: Liu, Xiaoxiao; Lv, Xue; Wu, Yeqing; Song, Jingjing; Wang, Xingguo; Zhu, Rong
• Is Part Of: Developmental and comparative immunology. Volume 109(2020)
• Publication Date: 2020-08
• Subjects: IRF7 -- Type I IFN -- Pelteobagrus fulvidraco -- Innate immune response -- Antiviral effect; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2020.103700
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Interferon regulatory factor 7 (IRF7) serves as a critical mediator in the regulation of type Ι interferon (IFN) response to invading pathogens. Here, an ortholog of IRF7 was characterized in yellow catfish ( Pelteobagrus fulvidraco ). The full-length cDNA of PfIRF7 consisted of 1516 bp encoding a polypeptide of 425 amino acids. PfIRF7 protein comprised a typical IRF structural architecture, including a DNA binding domain (DBD), an IRF association domain (IAD) and a serine-rich domain (SRD). PfIRF7 was expressed predominantly in the immune-related tissues and transcriptionally upregulated by PolyI:C, LPS, and Edwardsiella ictaluri . Ectopic expression of PfIRF7 led to activation of fish type I IFN promoters and induction of IFN and Vig1, thereby conferring a strong antiviral effect against spring viremia of carp virus (SVCV). Overall, the present data suggest that PfIRF7 may play an essential role in type I IFN response of yellow catfish. Highlights: Interferon regulatory factors 7 (IRF7) was identified and characterized from yellow catfish. PfIRF7 expression was be induced by PolyI:C, LPS, and Edwardsiella ictaluri. PfIRF7 stimulated fish IFN promoter activity and the expression of ISGs. Ectopic expression of PfIRF7 inhibited SVCV replication.