Identification of cofilin 1 as a candidate protein associated to mouse visual cortex plasticity. (13th July 2020)

• Record Type: Journal Article
• Authors: Bornia, Natalia; Taboada, Alfonso; Dapueto, Agustina; Rossi, Francesco Mattia
• Is Part Of: Neuroscience letters. Volume 731(2020)
• Publication Date: 2020-07-13
• Subjects: 2D bi-dimensional -- %Vol relative volume parameter -- AD adult -- APS ammonium persulfate -- AU acetic acid, urea -- AUT acetic acid, urea, Triton X-100 -- BSA bovine serum albumin -- CHAPS 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate -- CHCA α-cyano-4-hydroxycinnamic acid -- CHO Chinese hamster ovary -- CP critical period -- CTR control -- DTT dithiothreitol -- FLX fluoxetine -- H hippocampus -- HeLa human cervix adenocarcinoma -- HRP Horseradish peroxidase -- MCx motor cortex -- P postnatal age -- PBST Phosphate Buffer Saline solution containing Tween-20 -- SDS sodium dodecyl sulphate -- TFA trifluoroacetic acid -- VCx visual cortex; Visual cortex -- Plasticity -- Proteomics -- Basic proteins -- Histones -- Cofilin 1; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2020.135056
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: AUT/AU and AUT/AU bi-dimensional electrophoresis efficiently separates brain proteins. Cofilin 1 is dynamically regulated in the mouse visual cortex. Cofilin 1 is a candidate in the control of visual cortex plasticity. Abstract: In order to characterize the mechanisms controlling plasticity in the mouse visual cortex, we used, for the first time on brain samples, an unconventional proteomic approach to separate acid-extracted proteins by bi-dimensional electrophoresis (AUT/SDS or AUT/AU gels). The analysis was performed on high plasticity critical period young vs. low plasticity adult, and on fluoxetine-induced high plasticity vs. low plasticity untreated adult mice. Mass spectrometry allowed for the identification of 11 proteins that are differentially expressed between critical period and adult mice, and 5 between fluoxetine-treated and control adult mice. We then focused on cofilin 1, as the intensity level of the corresponding spot on 2D gels was higher in both high plasticity conditions. Western blot showed that cofilin 1 expression is dynamically regulated during postnatal life, reaching a peak at the critical period, and decreasing at adult stage, and that it increases in fluoxetine-treated vs. untreated adult mice. In summary, by using a 2D gel electrophoresis differential approach on basic proteins followed by mass spectrometry and immunoblot analysis, we identified cofilin 1 as a potential candidate controlling plasticity levels of the mouse visual cortex.