Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase. Issue 18 (6th September 2018)

• Record Type: Journal Article
• Authors: Proctor, Matthew S.; Chidgey, Jack W.; Shukla, Mahendra K.; Jackson, Philip J.; Sobotka, Roman; Hunter, C. Neil; Hitchcock, Andrew
• Is Part Of: FEBS letters. Volume 592:Issue 18(2018)
• Page Start: 3062
• Page End: 3073
• Publication Date: 2018-09-06
• Subjects: Arabidopsis -- chlorophyll -- chlorophyll synthase -- cyanobacteria -- high light‐inducible proteins -- YidC/Alb3/OxaI; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13222
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : In the model cyanobacterium Synechocystis sp. PCC 6803, the terminal enzyme of chlorophyll biosynthesis, chlorophyll synthase (ChlG), forms a complex with high light‐inducible proteins, the photosystem II assembly factor Ycf39 and the YidC/Alb3/OxaI membrane insertase, co‐ordinating chlorophyll delivery with cotranslational insertion of nascent photosystem polypeptides into the membrane. To gain insight into the ubiquity of this assembly complex in higher photosynthetic organisms, we produced functional foreign chlorophyll synthases in a cyanobacterial host. Synthesis of algal and plant chlorophyll synthases allowed deletion of the otherwise essential native cyanobacterial gene. Analysis of purified protein complexes shows that the interaction with YidC is maintained for both eukaryotic enzymes, indicating that a ChlG‐YidC/Alb3 complex may be evolutionarily conserved in algae and plants. Abstract :