Syndecan‐4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition. Issue 18 (7th September 2018)

• Record Type: Journal Article
• Authors: Keller‐Pinter, Aniko; Szabo, Kitti; Kocsis, Tamas; Deak, Ferenc; Ocsovszki, Imre; Zvara, Agnes; Puskas, Laszlo; Szilak, Laszlo; Dux, Laszlo
• Is Part Of: FEBS letters. Volume 592:Issue 18(2018)
• Page Start: 3139
• Page End: 3151
• Publication Date: 2018-09-07
• Subjects: myoblast -- myostatin -- syndecan‐4; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13227
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Myostatin, a TGF‐β superfamily member, is a negative regulator of muscle growth. Here we describe how myostatin activity is regulated by syndecan‐4, a ubiquitous transmembrane heparan sulfate proteoglycan. During muscle regeneration the levels of both syndecan‐4 and promyostatin decline gradually after a sharp increase, concurrently with the release of mature myostatin. Promyostatin and syndecan‐4 co‐immunoprecipitate, and the interaction is heparinase‐sensitive. ShRNA‐mediated silencing of syndecan‐4 reduces C2C12 myoblast proliferation via blocking the progression from G1‐ to S‐phase of the cell cycle, which is accompanied by elevated levels of myostatin and p21(Waf1/Cip1), and decreases in cyclin E and cyclin D1 expression. Our results suggest that syndecan‐4 functions as a reservoir for promyostatin regulating the local bioavailability of mature myostatin. Abstract :