Roseovarius arcticus sp. nov., a bacterium isolated from Arctic marine sediment. Issue 3 (2nd March 2020)

• Record Type: Journal Article
• Authors: Lu, Lu; Zhang, Yumin; Peng, Xiaoya; Liu, Jia; Qin, Kun; Peng, Fang
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 70:Issue 3(2020)
• Page Start: 2072
• Page End: 2078
• Publication Date: 2020-03-02
• Subjects: Roseovarius arcticus -- arctic -- sediment -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.004018
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : An aerobic, Gram-stain-negative, motile, rod or long-rod-shaped bacterial isolate, strain MK6-18 T, was isolated from a marine sediment sample from Kongsfjorden, Arctic. The bacterium grew optimally at 20 °C, pH 7.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MK6-18 T belonged to the genus Roseovarius . Its closest phylogenetic neighbour was Roseovarius nanhaiticus NH52J T showing 96.97 % 16S rRNA gene sequence similarity. The genome of strain MK6-18 T is 4.2 Mb long in size with a G+C content of 59.5 mol%. The average nucleotide identity value between the genomes of strain MK6-18 T and Roseovarius nanhaiticus NH52J T, was 78.0 %. Similar to other species of the genus Roseovarius, strain MK6-18 T had ubiquinone 10 as the predominant ubiquinone and C12 : 0, C16 : 0, summed feature 3 (C16 : 1 ω7c / ω6 c ) and summed feature 8 (C18 : 1 ω7c / ω6 c ) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; one unidentified polar lipid, one unidentified aminolipid and one unidentified lipid were also detected. This is the first time that a member of the genus Roseovarius has been isolated from the Arctic, which may promote the study of the distribution characteristics and environmental adaptability of this genus. On the basis of the data provided here, strain MK6-18 T should be classed as representing a novel species of the genus Roseovarius, for which the name Roseovarius arcticus sp. nov. is proposed. The type strain is MK6-18 T (=CCTCC AB 2018219 T =KCTC 72187 T ).